PCR Reagents Products & Reviews

The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.

Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

PCR Water is a molecular biology grade product suitable for sample dilution, and use as an ultra-pure diluent for PCRs and other molecular biology reactions.

Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA

Universal 18S rDNA real-time PCR assay for sensitive detection of fungal DNA

Easy-to-use, one-component reagent for internal labeling of oligonucleotide probes to be detected on the LightCycler® and LightTyper Instruments. Application The SimpleProbe 519 Labeling Reagent is used for the internal labeling of oligonucleotide probes that are to be detected at  519 nm, for example, when used as mutation detection probes on the LightCycler® and LightTyper Systems following the SimpleProbe probe principle.…

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

Precision Blue™ Real-Time PCR Dye, 55 µl (500 x 20 µl reactions)

High-quality, molecular-grade dNTPs. Kapa offers premium grade dNTPs for PCR. KAPA dNTP Mix contains dATP, dCTP, dGTP, and dTTP supplied at a concentration of 10 mM each.   Applications: Standard PCR qPCR cDNA synthesis DNA sequencing DNA labeling Microarrays

Double-quenched probes that offer improved quenching over longer probe sequences

Sensitive Quantitation of Single-Stranded DNARequires less DNA than spectrophotometry

cGMP-Grade Solution of dNTPs Pretested in PCR- Two concentrations available: 10mM and 25mM

PCR oligonucleotide primer sets consisting of 16  primers  for amplification and 16 primers  for cloning of human IgG and IgM heavy and light chain variable domain coding regions for generating universal, patient or antibody-specific scFv phage-display libraries. 

PCR oligonucleotide primer sets consisting of 25 primers for amplification and 25 primers for cloning of mouse IgG heavy and light chain variable domain coding regions for generating universal or antibody-specific scFv phage-display libraries.

98-100%

High purity, well characterized trehalose in powder or solution

HiScript III U+ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. 

CPG used to add the non-fluorescent quencher BHQ-1 dye to the 3' end of an oligonucleotide.

This ready-to-use nucleotide mix is a premixed solution of multiple PCR-grade nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration of 10 mM). This mix is optimized for use in all types of amplification reactions and primer-extension reactions. PCR RT-PCR cDNA synthesis Primer extension Benefits Improve yield and performance Choose a convenient ready-to-use mix of all 4 nucleoti…

The Deoxynucleoside Triphosphate Set contains 4 individual vials of dATP, dCTP, dGTP, and dTTP, each as a 100 mM solution of the Na-salt. PCR-grade nucleotides are optimized for use in all types of amplification reactions and primer-extension reactions. Product Description PCR-Grade Nucleotides from Roche Applied Science are specially manufactured and purified to the highest possible chemical purity. PCR-Grade Nucleoti…

The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides.FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incuba…

The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult…

The enzyme dNTPack comprises the GC-RICH PCR System and a ready-to-use solution of PCR-Grade Nucleotides.The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior perfo…