PCR Reagents
Optimized primers for different human miRNA that were selected based upon their roles in cancer and development. Detects mature miRNA Detects human, mouse, rat, worm and other species Highly-specific sequences assure single nucleotide discrimination
Optimized primers for different human miRNA that were selected based upon their roles in cancer and development. Detects mature miRNA Detects human, mouse, rat, worm and other species Highly-specific sequences assure single nucleotide discrimination
Optimized primers for different human miRNA that were selected based upon their roles in cancer and development. Detects mature miRNA Detects human, mouse, rat, worm and other species Highly-specific sequences assure single nucleotide discrimination
Optimized primers for different human miRNA that were selected based upon their roles in cancer and development. Detects mature miRNA Detects human, mouse, rat, worm and other species Highly-specific sequences assure single nucleotide discrimination
Optimized primers for different human miRNA that were selected based upon their roles in cancer and development. Detects mature miRNA Detects human, mouse, rat, worm and other species Highly-specific sequences assure single nucleotide discrimination
The Prime-It Fluor fluorescence labeling kit generates directly fluoresceinated DNA probes using random 9-mer primers and Exo-Klenow to incorporate fluor-12-dUTP into the probe fragments. Generates high-specific-activity probes in 2 minutes Fluorescently labeled probes also available Produces labeled fragments 400 bp to 10 kb nucleotides in length Performs equally well with [32P]dATP and [32P]dCTP Performs well w…
The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.
The passive reference dye can be added to fluorescence-based quantitative PCR (qPCR) assays to compensate for non-PCR related variations in fluorescence. Product comes in 10 vials of 100 µL. 1mM for controlling the final dye concentration.
The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.
Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.
Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.
The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.
Easy-to-use, one-component reagent for internal labeling of oligonucleotide probes to be detected on the LightCycler® and LightTyper Instruments. Application The SimpleProbe 519 Labeling Reagent is used for the internal labeling of oligonucleotide probes that are to be detected at 519 nm, for example, when used as mutation detection probes on the LightCycler® and LightTyper Systems following the SimpleProbe probe principle.…
High-quality, molecular-grade dNTPs. Kapa offers premium grade dNTPs for PCR. KAPA dNTP Mix contains dATP, dCTP, dGTP, and dTTP supplied at a concentration of 10 mM each. Applications: Standard PCR qPCR cDNA synthesis DNA sequencing DNA labeling Microarrays
PCR oligonucleotide primer sets consisting of 16 primers for amplification and 16 primers for cloning of human IgG and IgM heavy and light chain variable domain coding regions for generating universal, patient or antibody-specific scFv phage-display libraries.
PCR oligonucleotide primer sets consisting of 25 primers for amplification and 25 primers for cloning of mouse IgG heavy and light chain variable domain coding regions for generating universal or antibody-specific scFv phage-display libraries.
Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA
Universal 18S rDNA real-time PCR assay for sensitive detection of fungal DNA
HiScript III U+ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.
This ready-to-use nucleotide mix is a premixed solution of multiple PCR-grade nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration of 10 mM). This mix is optimized for use in all types of amplification reactions and primer-extension reactions. PCR RT-PCR cDNA synthesis Primer extension Benefits Improve yield and performance Choose a convenient ready-to-use mix of all 4 nucleoti…
The Deoxynucleoside Triphosphate Set contains 4 individual vials of dATP, dCTP, dGTP, and dTTP, each as a 100 mM solution of the Na-salt. PCR-grade nucleotides are optimized for use in all types of amplification reactions and primer-extension reactions. Product Description PCR-Grade Nucleotides from Roche Applied Science are specially manufactured and purified to the highest possible chemical purity. PCR-Grade Nucleoti…
The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides.FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incuba…
The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult…
The enzyme dNTPack comprises the GC-RICH PCR System and a ready-to-use solution of PCR-Grade Nucleotides.The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior perfo…
