PCR Reagents Products & Reviews

The Prime-It Fluor fluorescence labeling kit generates directly fluoresceinated DNA probes using random 9-mer primers and Exo-Klenow to incorporate fluor-12-dUTP into the probe fragments.  Generates high-specific-activity probes in 2 minutes  Fluorescently labeled probes also available  Produces labeled fragments 400 bp to 10 kb nucleotides in length  Performs equally well with [32P]dATP and [32P]dCTP  Performs well w…

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

The passive reference dye can be added to fluorescence-based quantitative PCR (qPCR) assays to compensate for non-PCR related variations in fluorescence. Product comes in 10 vials of 100 µL. 1mM for controlling the final dye concentration.

The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.

Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.

A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

Sensitive Quantitation of Single-Stranded DNARequires less DNA than spectrophotometry

cGMP-Grade Solution of dNTPs Pretested in PCR- Two concentrations available: 10mM and 25mM

Modifications that enhance the performance of oligonucleotides

PCR Water is a molecular biology grade product suitable for sample dilution, and use as an ultra-pure diluent for PCRs and other molecular biology reactions.

Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA

Universal 18S rDNA real-time PCR assay for sensitive detection of fungal DNA

Easy-to-use, one-component reagent for internal labeling of oligonucleotide probes to be detected on the LightCycler® and LightTyper Instruments. Application The SimpleProbe 519 Labeling Reagent is used for the internal labeling of oligonucleotide probes that are to be detected at  519 nm, for example, when used as mutation detection probes on the LightCycler® and LightTyper Systems following the SimpleProbe probe principle.…

High-quality, molecular-grade dNTPs. Kapa offers premium grade dNTPs for PCR. KAPA dNTP Mix contains dATP, dCTP, dGTP, and dTTP supplied at a concentration of 10 mM each.   Applications: Standard PCR qPCR cDNA synthesis DNA sequencing DNA labeling Microarrays

PCR oligonucleotide primer sets consisting of 16  primers  for amplification and 16 primers  for cloning of human IgG and IgM heavy and light chain variable domain coding regions for generating universal, patient or antibody-specific scFv phage-display libraries. 

PCR oligonucleotide primer sets consisting of 25 primers for amplification and 25 primers for cloning of mouse IgG heavy and light chain variable domain coding regions for generating universal or antibody-specific scFv phage-display libraries.

98-100%

High purity, well characterized trehalose in powder or solution