PCR Reagents Products & Reviews

Compact PCR probes for detecting difficult target sequences

Custom design your oligonucleotide according to your specifications. Our probes and primers are manufactured at unmatched quality under ISO 9001 and 13485 compliance. Whether you need RUO or GMP grade oligos, choose from a vast selection of modifications to customize your oligos for your application.

SARS-CoV-2 Variant ValuPanel™ assays consist of separately delivered probes and primers that are designed for qualitative detection of specific SARS-CoV-2 mutations by genotyping using reverse transcription-polymerase chain reaction (RT-PCR). 

Effective genomic surveillance of SARS-CoV-2 variants begins with the right tools 

IDT offers a wide array of primer and probe sets, as well as plasmid controls, for the identification of SARS-CoV-2 (2019-nCoV). Primers and probes are manufactured in a template-free environment and certified template-free to cycle 45 by NTC testing.

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

PerfeCTa qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

DNA-on Demand with benchtop convenience and same-day results.

A ready-to-use Rt-PCR Kit for Detection of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19).

Choice Taq Mastermix is a 2X reaction mixture. It is ready-to-go and it only requires addition of template, primers, and water.

The repliQa HiFi ToughMix is a 2x, ready-to-use master mix that contains all the components for high fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies. This unique, next generation master mix provides 90x higher fidelity compared to Taq, while reducing time to PCR results by 2-3x. The extreme speed is enabled by extension times as fast as 1-10 kb/sec depending on targ…

Thermo Scientific Anchored Oligo-dT primers are specifically designed for synthesising long cDNAs from mRNAs carrying poly-A tails. Since this primer only anneals to the poly-A tail junction of the mRNA template, it eliminates transcription through the poly-A tail, providing more effective cDNA synthesis.

dNTP mixes – supplied in a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP. Thermo Scientific produces extremly pure dNTPs, and the company is one of only a few primary manufacturers in the industry. Thermo Scientific dNTPs are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually as nucleotide mixes at convenient concentrations for direct use in PCR, reverse transcription, label…

Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex Primer Pools deliver comprehensive coverage of Sanger miRBase v10, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution. Optional Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs en…

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The…

These reactions buffers contain the optimal magnesium concentration for efficient PCR.  Ideal for optimal PCR performance  Standard 10x formulation  Optimized for use with specific PCR enzymes

Thermo Scientific ThermoPrime Taq DNA Polymerase with 10X ReddyMix PCR Buffer. This unique buffer has an inert red dye and a density reagent added. After thermal cycling, a sample of the PCR mix may be removed and loaded directly onto an agarose gel without the addition of loading dye. ReddyMix has no adverse effect on the outcome of PCR; yields are the same as with standard ThermoPrime Taq DNA Polymerase. Convenient Master Mi…

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime Taq DNA Polymerase, High Fidelity, you'll get: • The…

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Double-quenched probes that offer improved quenching over longer probe sequences

CPG used to add the non-fluorescent quencher BHQ-1 dye to the 3' end of an oligonucleotide.

Precision Blue™ Real-Time PCR Dye, 55 µl (500 x 20 µl reactions)

PCR Water is a molecular biology grade product suitable for sample dilution, and use as an ultra-pure diluent for PCRs and other molecular biology reactions.