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NanoBRET™ TE Intracellular Kinase Assays

Promega Corp.

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tra…

4.7/5.0
|22 Reviews
  • Best New Drug Discovery & Development Product of the Year
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6 × 5 LC-MS/MS Peptide Reference Mix

Promega Corp.

A Complete Solution for Monitoring LC and MS Instrument Quality Control and Performance. In combination with an accompanying software tool, the 6 × 5 LC-MS/MS Peptide Reference Mix provides a convenient way to assess LC column performance and MS instrument parameters, including sensitivity and dynamic range. Consists of 30 peptides; 6 sets of 5 isotopologues of the same peptide sequence, differing only in the number o…

5.0/5.0
|1 Review
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Digitonin

Promega Corp.

Digitonin can be used in cell viability and cytotoxicity assays. It is a detergent solution useful for permeabilizing cells and for creating a cytotoxicity chemistry positive control. Supplied at 20mg/ml in DMSO

5.0/5.0
|1 Review
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Nano-Glo® Live Cell Assay System

Promega Corp.

Measures NanoLuc® Luminescence in Living Cells Substrate and buffer optimized to enhance stability Use to detect NanoBiT® protein complementation or NanoLuc® reporter activity Monitors luminescence at a single time point or continuously for up to 2 hours without compromising cell viability.

4.7/5.0
|1 Review
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Pfu DNA Polymerase

Promega Corp.

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

5.0/5.0
|1 Review
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CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Promega Corp.

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS(a)] and an electron coupling reagent (phenazine ethos…

5.0/5.0
|2 Reviews
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pGEM®-T Easy Vector Systems

Promega Corp.

The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.   They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the…

4.8/5.0
|8 Reviews
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