UHPLC and HPLC Products & Reviews

High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Ultra IBD, 3 µm, 100 x 2.1 mm HPLC column

Restek Corp.

The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, these columns are designed for selective and reliable HPLC applications, for mixed polar and nonpolar compounds.

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Thermo Scientific™ Acclaim™ Trinity P1 LC Column

Thermo Fisher Scientific

Acclaim™ Trinity P1 is a unique, high-efficiency, silica-based column designed for pharmaceutical applications such as simultaneous separation of pharmaceutical drug substances and their counterions. his new column, based on nanopolymer silica hybrid (NSH) technology, provides multiple retention mechanisms including reversed-phase, anion-exchange, and cation-exchange. As a result, its selectivity can be easily opti…

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Thermo Scientific™ Acclaim™ Explosives LC Column

Thermo Fisher Scientific

The Thermo Scientific Acclaim™ Explosives E1 (5 µm) and E2 (5, 3 and 2.2 μm) silica-based reversed-phase columns are designed to resolve all 14 explosives listed in EPA SW-846 Method 8330: Nitroaromatics and Nitramines by HPLC. Their unique chemistries deliver superior resolution with complementary selectivities. The E1 is recommended for use as a direct replacement for ODS columns for the primary analysis.The E2 may be used a…

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Thermo Scientific™ Acclaim™ 300 C18 Protein and Peptide Column

Thermo Fisher Scientific

High-Resolution Separation of Proteins and Peptides The Acclaim® 300 features 3 µm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residua…

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Thermo Scientific™ DNASwift™ SAX-1S Oligonucleotide Columns

Thermo Fisher Scientific

The DNASwift™ SAX-1S is a strong anion-exchange column that provides exceptionally high resolution for the purification of oligonucleotides.The exceptionally high resolution provides superior purity and yields compared to other ion-exchange or reversed-phase columns available. The DNASwift column is ideal for therapeutic and diagnostic research, which have very high purity and yield oligonucleotides purification requirements.T…

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Thermo Scientific™ Acclaim™ PepMap™ 100 C18 Protein and Peptide Column

Thermo Fisher Scientific

The Thermo Scientific Acclaim™ PepMap™ 100 C18 column sets the benchmark in peptide separation.The PepMap100 C18 column is available in 3 or 5 µm particle sizes, with 100 Å pore sizes, and in nano, capillary, and micro formats. nanoViper fingertight fittings allow for easy column installation.Column Details: Chemistry: C18 Particle Size: 3, 5 μm Pore Size: 100 Å Length: 5, 15, 25, 50 mm ID: 75, 300 μm, 1.0 mm

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Thermo Scientific™ MAbPac™ SCX-10 Monoclonal Antibody Analysis Column

Thermo Fisher Scientific

MAbPac™ SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with…

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Thermo Scientific™ ProPac™ SAX and WAX Protein Column

Thermo Fisher Scientific

High-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of ProteinsThe ProPac® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anio…

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Thermo Scientific™ ProPac™ SCX and WCX Protein Column

Thermo Fisher Scientific

Strong and Weak Cation-Exchange Columns for High-Resolution Separation of Proteins and MAbsThe Thermo Scientific ProPac™ SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resoluti…

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