High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
BetaMax Acid Columns o High surface area area and bonded phase coverage for maximum retention, even in high percentage organic conditionso Chemistries for acidic compounds Product detail: For alternative selectivity to traditional alkyl phases, we recommend you try the polar embedded phase BetaMax columns. The stationary phase contains urea groups embedded within the C12 chain, which extra interactions with samples to…
BETASIL C18 Columnso Broad range of particle sizes and phases for wide method development optionso High surface area and phase coverage for reproducibility and sharp peak shapes Product Detail BETASIL columns are based on 100Å pore size, fully deactivated type B silica to ensure sharp peaks and excellent reproducibility for the analysis of a wide range of compounds. The 100Å pore size gives a higher concentration of statio…
BetaBasic 4 Columnso Excellent peak shapes for basic compoundso Superb stability at both low and high pH Product detail:BetaBasic 4 columns are based on a 150Å pore size, fully deactivated type B pure silica to ensure sharp peaks and excellent reproducibility for the analysis of basic compounds. Due to stable bonding chemistry and excellent surface coverage, BetaBasic 4 columns show excellent peak shape for basic compound…
Hyperbond™ is a cost effective alternative to µBondapak (a trademark of Waters Corporation). It is based on an irregular silica with 10µm particle size.
o 100% porous graphitic carbon with very different selectivity to reversed-phase silica columnso Exceptional retention of very polar compoundso Separation of structurally similar compoundso Stable from pH 1 to 14 at high temperatures in a wide range of solvents Product detail: Hypercarb is a unique material for HPLC manufactured by Thermo Hypersil-Keystone. Hypercarb consists of fully porous spherical carbon particle…
o Separate hydrophobic and ionic compounds in a single runo Select from two mixed mode phases, using either cation or anion exchange combined with C18o Retention of compounds is optimized using mobile phase pH, ionic strength and percent organic Product detail: Hypersil Duet columns are an ideal choice for unknown compounds and metabolism studies, as they can separate hydrophobic and ionic compounds in a single analysis…
The Hypersil SAX is recommended for analysis of small organic molecules and organic acids. Product detail: Hypersil SAX is a strong anion exchange column, which is typically used for analysis of small organic molecules and organic acids. As the columns are based on the first generation classical 120Å pore size silica they are not recommended for analysis of biomolecules. For new methods we recommend trying our latest ion…
Thermo Electron Hot and Cool Pockets™ offer unbeatable value in coulmn temperature control. The pockets are small and light weight yet offer precise temperature control. Hot pockerts heat from 5 to 85 oC with a temperature accuracy of ±2oC over the entir range, and a temperature stability of ±0.1Oc. Hot Pockets™ and Cool Pockets™ are not available within the European Union at the present time. We apologise for any inconvenien…
A new screw cap that has been introduced for HP users, to enable them to use a screw top vial on a robotic arm autosampler.
The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…
The DE Midi range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, primarily in Development, who are looking purify samples from 5 grams to 1,000 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…
Scaling up is easier when using an HPLC media that provides near identical performance across all particle sizes and with increases in column diameter. The separation characteristics of each Phenomenex media offer unique selectivity for each type of application and significant production gains for maximum loadability and process economy. Achieve identical performance from analytical development to large process scale. Our Luna…
SCAT solvent safety systems have been designed to bring solvent safety to the laboratory. The specially designed caps for both solvent reservoirs and waste containers fit virtually any sized container. Certified by TUV in Germany these safety systems af guaranteed to protect your health in the laboratory. Recent legislation has meant the use of solvent safety systems are mandatory in a number of EU countries and several EU m…
With a fresh and customer focussed approach to Polymer Analysis, PSS is Germany's leading authority in Gel Permeation Chromatography, GPC and SEC. The most comprehensive range of products for standards, columns instruments and software coupled with more than twenty years experience in delivering customer oriented results. A complete range of standards for organic and aqueous GPC Columns for organic separations Columns f…
ELSD detectors - 3 models to choose from Model 100 ELSD – Simple OperationModel 300S ELSD – Advanced ControlModel 400 ELSD – Sophisticated Sytem Management Highest dynamic range 4.5 orders of magnitude Equal response between analytes High sensitivity even for semi-volatiles Independantly control the temperature of the nebuliser and drift tube
AZUR 4.0 The latest version of chromatography data system for stand-alone acquisition and analysis. Many new features including enhanced reporting capabilities and the new 24 bit USB interface. USB or PCI interface up to 4 independant channels Simple user interface Advanced functionality Low cost academic version
Proteema is the right choice for the separation of all kinds of biomolecules including proteins and gelatines. The columns are silica based and can be used with eluents up to a pH value of 7. If the application requires separations at higher pH in more ‘basic’ eluents, PSS SUPREMA a polymer based material is recommended. Generally the pore-size distribution of silica based columns is smaller than that of polymeric columns, whi…
1 to 8 channels online vacuum degasser Low cost replacement degassing units to replace broken units or helium sparging. You can easily improve chromatography by degassing solvents. Three ranges in one! Degasys - low cost unit with options for prep HPLC Degasys Populaire - routing unit of choice for most applications Degasys Ultimate - high quality unit most often used for low volume HPLC Low cost Degasys degassers…
Advantec Japan, manufactures two great fraction collectors which can be used for both HPLC and other types of fraction collecting. Both units offer exceptional flexibility from the rugged x-y-z operation High quality Construction Wide choice of racks Software compatible Large sample capacity Prep option Collect by time, vol, drop or peak signal detection CHF100SA Routine Collector A low cost Fraction Collecto…
ReadyCal Kits PSS ReadyCal Standards are polymer cocktails preweighted into auto-sampler vials. Each vial has three or four polymers of the same type with different molar masses. Carefully selected molar masses are placed into each vial so that the polymer traces are baseline separated. A ReadyCal Kit allows you to prepare fast and reproducible 8 - 12 point calibration curve without the mess of weighing the samples. Just add s…
Phenogel™ Gel Permeation Chromatography Columns for size exclusion are available in seven different pore sizes ranging from 50 Å to 10 6 Å, and a linear bed configuration. Pore size distribution and pore volume are closely controlled parameters in the manufacturing process, accounting for high resolution, tight linear calibration curves and excellent column-to-column reproducibility.Phenogel GPC columns offer high cross-linkag…
Syringes used to fill a loop on a sample injection valve have blunt, smooth ends. The end of the needle must contact the bottom of the valve’s fitting detail uniformly and seal on the outside of the tip to deliver sample with any repeatability. All VICI Precision Sampling syringes for valve injections have smooth, burr-free ends that fit the valve fitting details perfectly. The standard HPLC syringe is our basic C-160 with a 2…
Syringes used to fill a loop on a sample injection valve have blunt, smooth ends. The end of the needle must contact the bottom of the valve’s fitting detail uniformly and seal on the outside of the tip to deliver sample with any repeatability. All VICI Precision Sampling syringes for valve injections have smooth, burr-free ends that fit the valve fitting details perfectly. The standard HPLC syringe is our basic C-160 with a 2…
Hollow, flow-through plunger with integrated 3-way valve designed for liquid and gases, with a push button on/off valve at the front end. The new Precision Sampling hollow plunger syringe with two integrated valves opens a whole new world of application possibilities: Dispense a viscous sample more easily by introducing it into the rear of the syringe instead of drawing it up through the needle. Eliminate sample-to-sample cros…
The Dual Layer PEEK tubing eliminates any concern that a critical sample stream could be contaminated by pigments used to color code the tubing. The pigmented layer surrounds a separate but integrally-bonded inner layer of natural PEEK. The 1/16" OD tubing is available in standard sizes, or in OEM quantities with any ID you specify, from .002" to .030". The ID tolerance of ±.001" is equivalent to or better than that of tubing…
