High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The Waters IdentityE High Definition Proteomics system defines the new standard for rigorous protein identification. The IdentityE High Definition Proteomics System is an UltraPerformance LC® (UPLC)/High-Bandwidth Mass Spectrometry (MS E) system solution suitable for confident protein identification designed to meet stringent requirements as outlined by leading scientific journals.The new Waters IdentityE High Definition Pro…
The ACQUITY UPLC® Fluorescence (FLR) Detector brings unrivaled sensitivity and selectivity to UPLC®. The detector’s innovative flow-cell design, low-noise electronics and support for high-speed data rates are perfectly suited to the efficient separations of the ACQUITY UPLC's advanced fluidics technology. Target applications include PAHs, environmental contaminants, vitamins, aflatoxins and pharmaceuticals. Ultra Performance i…
SymmetryShield™ Columns feature Waters' patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes at improves overall peak shape. Additionally, by placing the embedded polar group close to the silica surface, the activity of the surface silanols is further reduced. This imparts selectivity and retention that is different compared to the Symmetry C18 ligand. Embedded polar…
Polynuclear Aromatic Hydorcarbons (PAHs) are among the most frequently monitored environmental contaminants. Waters PAH columns are optimized for the HPLC analysis of PAHs. These columns achieve baseline resolution and excellent peak symmetry for 16 different target analytes in less than 25 minutes, while employing a simple water/acetonitrile binary gradient method. The resolving power of the PAH columns provides superior pe…
The popular Bondapak® C18 chemistry and Porasil® silica packing material are available in three different particle sizes, 10, 15-20, 37-55 micron, providing easy transfer of chromatography methodology from one particle size to another. You can utilize your existing 10 micron Bondapak or Porasil chromatography as a starting point for scale-up or, if you are developing a new purification protocol, investigate which preparative…
These columns provide accurate and precise molecular weight calculations in under 7 minutes! - Fast, accurate and precise molecular weight determinations - Increase sample throughput - Greatly decrease solvent consumption and disposal
The Envirogel high-efficiency GPC cleanup columns are specifically designed to remove low volatility, high molecular weight interferences, such as lipids and natural resins, from environmental samples as specified in EPA Method 3640A. In the past, the cleanup procedure for environmental samples was performed on low-efficiency GPC columns based on packing particle diameters of 37-75 micron (200 to 400 mesh) Bio-Beads S-X resins…
For applications where the sample is neither volume or concentration limited, Waters offers 1 and 2mm Internal Diameter columns of various lengths for use on traditional HPLC MS inlets.As with Waters array of NanoEase™ columns, the same Waters reversed-phase packings are also available in these two column internal diameter offerings. Chemistry choices include: XBridge® Columns, CORTECS® Columns, XSelect Columns, Atlantis® Pola…
MassPREP™ On-Line Desalting Cartridges are an effective tool for the successful desalting of acidic, basic, and large globular proteins. The overall MassPREP™ desalting strategy provides an easy, fast (< 3 minutes), and reproducible approach for the effective desalting of proteins stored in physiological salt buffers prior to high resolution ESI-MS structural analysis.
The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…
The DE Midi range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, primarily in Development, who are looking purify samples from 5 grams to 1,000 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…
Scaling up is easier when using an HPLC media that provides near identical performance across all particle sizes and with increases in column diameter. The separation characteristics of each Phenomenex media offer unique selectivity for each type of application and significant production gains for maximum loadability and process economy. Achieve identical performance from analytical development to large process scale. Our Luna…
SCAT solvent safety systems have been designed to bring solvent safety to the laboratory. The specially designed caps for both solvent reservoirs and waste containers fit virtually any sized container. Certified by TUV in Germany these safety systems af guaranteed to protect your health in the laboratory. Recent legislation has meant the use of solvent safety systems are mandatory in a number of EU countries and several EU m…
With a fresh and customer focussed approach to Polymer Analysis, PSS is Germany's leading authority in Gel Permeation Chromatography, GPC and SEC. The most comprehensive range of products for standards, columns instruments and software coupled with more than twenty years experience in delivering customer oriented results. A complete range of standards for organic and aqueous GPC Columns for organic separations Columns f…
ELSD detectors - 3 models to choose from Model 100 ELSD – Simple OperationModel 300S ELSD – Advanced ControlModel 400 ELSD – Sophisticated Sytem Management Highest dynamic range 4.5 orders of magnitude Equal response between analytes High sensitivity even for semi-volatiles Independantly control the temperature of the nebuliser and drift tube
AZUR 4.0 The latest version of chromatography data system for stand-alone acquisition and analysis. Many new features including enhanced reporting capabilities and the new 24 bit USB interface. USB or PCI interface up to 4 independant channels Simple user interface Advanced functionality Low cost academic version
Proteema is the right choice for the separation of all kinds of biomolecules including proteins and gelatines. The columns are silica based and can be used with eluents up to a pH value of 7. If the application requires separations at higher pH in more ‘basic’ eluents, PSS SUPREMA a polymer based material is recommended. Generally the pore-size distribution of silica based columns is smaller than that of polymeric columns, whi…
1 to 8 channels online vacuum degasser Low cost replacement degassing units to replace broken units or helium sparging. You can easily improve chromatography by degassing solvents. Three ranges in one! Degasys - low cost unit with options for prep HPLC Degasys Populaire - routing unit of choice for most applications Degasys Ultimate - high quality unit most often used for low volume HPLC Low cost Degasys degassers…
Advantec Japan, manufactures two great fraction collectors which can be used for both HPLC and other types of fraction collecting. Both units offer exceptional flexibility from the rugged x-y-z operation High quality Construction Wide choice of racks Software compatible Large sample capacity Prep option Collect by time, vol, drop or peak signal detection CHF100SA Routine Collector A low cost Fraction Collecto…
ReadyCal Kits PSS ReadyCal Standards are polymer cocktails preweighted into auto-sampler vials. Each vial has three or four polymers of the same type with different molar masses. Carefully selected molar masses are placed into each vial so that the polymer traces are baseline separated. A ReadyCal Kit allows you to prepare fast and reproducible 8 - 12 point calibration curve without the mess of weighing the samples. Just add s…
Phenogel™ Gel Permeation Chromatography Columns for size exclusion are available in seven different pore sizes ranging from 50 Å to 10 6 Å, and a linear bed configuration. Pore size distribution and pore volume are closely controlled parameters in the manufacturing process, accounting for high resolution, tight linear calibration curves and excellent column-to-column reproducibility.Phenogel GPC columns offer high cross-linkag…
Syringes used to fill a loop on a sample injection valve have blunt, smooth ends. The end of the needle must contact the bottom of the valve’s fitting detail uniformly and seal on the outside of the tip to deliver sample with any repeatability. All VICI Precision Sampling syringes for valve injections have smooth, burr-free ends that fit the valve fitting details perfectly. The standard HPLC syringe is our basic C-160 with a 2…
