High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Proteema is the right choice for the separation of all kinds of biomolecules including proteins and gelatines. The columns are silica based and can be used with eluents up to a pH value of 7. If the application requires separations at higher pH in more ‘basic’ eluents, PSS SUPREMA a polymer based material is recommended. Generally the pore-size distribution of silica based columns is smaller than that of polymeric columns, whi…
1 to 8 channels online vacuum degasser Low cost replacement degassing units to replace broken units or helium sparging. You can easily improve chromatography by degassing solvents. Three ranges in one! Degasys - low cost unit with options for prep HPLC Degasys Populaire - routing unit of choice for most applications Degasys Ultimate - high quality unit most often used for low volume HPLC Low cost Degasys degassers…
Advantec Japan, manufactures two great fraction collectors which can be used for both HPLC and other types of fraction collecting. Both units offer exceptional flexibility from the rugged x-y-z operation High quality Construction Wide choice of racks Software compatible Large sample capacity Prep option Collect by time, vol, drop or peak signal detection CHF100SA Routine Collector A low cost Fraction Collecto…
ReadyCal Kits PSS ReadyCal Standards are polymer cocktails preweighted into auto-sampler vials. Each vial has three or four polymers of the same type with different molar masses. Carefully selected molar masses are placed into each vial so that the polymer traces are baseline separated. A ReadyCal Kit allows you to prepare fast and reproducible 8 - 12 point calibration curve without the mess of weighing the samples. Just add s…
200A pore size; available in 3.0 and 4.6 mm ids, and lengths from 50 - 250 mm. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process. Build on the large 200A pore size silica, high liquid density delivers up to 40% faster separations without sacrif…
Agilent Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases.
Syringes used to fill a loop on a sample injection valve have blunt, smooth ends. The end of the needle must contact the bottom of the valve’s fitting detail uniformly and seal on the outside of the tip to deliver sample with any repeatability. All VICI Precision Sampling syringes for valve injections have smooth, burr-free ends that fit the valve fitting details perfectly. The standard HPLC syringe is our basic C-160 with a 2…
Syringes used to fill a loop on a sample injection valve have blunt, smooth ends. The end of the needle must contact the bottom of the valve’s fitting detail uniformly and seal on the outside of the tip to deliver sample with any repeatability. All VICI Precision Sampling syringes for valve injections have smooth, burr-free ends that fit the valve fitting details perfectly. The standard HPLC syringe is our basic C-160 with a 2…
Hollow, flow-through plunger with integrated 3-way valve designed for liquid and gases, with a push button on/off valve at the front end. The new Precision Sampling hollow plunger syringe with two integrated valves opens a whole new world of application possibilities: Dispense a viscous sample more easily by introducing it into the rear of the syringe instead of drawing it up through the needle. Eliminate sample-to-sample cros…
The Dual Layer PEEK tubing eliminates any concern that a critical sample stream could be contaminated by pigments used to color code the tubing. The pigmented layer surrounds a separate but integrally-bonded inner layer of natural PEEK. The 1/16" OD tubing is available in standard sizes, or in OEM quantities with any ID you specify, from .002" to .030". The ID tolerance of ±.001" is equivalent to or better than that of tubing…
The Advantages at a Glance Ready to use columns No more laborious cleaning of Glass Columns Easy handling Reduced Costs- the more samples the more cost effective Certified Columns Excellent Performance The determination of the hydrocarbon oil index in surface, industrial and process water in compliance with EN ISO 9377-2 (H53) is daily routine in many laboratories. Using intelligent sample preparation, time and costs for the a…
"Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5. Columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH.…
Designed for Rapid Resolution and Ultra Performance LC Systems • Communicates with ChemStation via an exclusive SofTA Driver. • Superior HPLC Detection to Replace or Complement your Current Detection. • Sensitive Detection for a Wide Variety of Chemical Classes and Structures. • Easy to Use, Fast Start-up, No Consumable Parts, Low Cost of Operation. • Precise Vapor Phase Control with SofTA Exclusive Thermo-Split™ Technology. •…
Biochrom instruments are supplied complete with the analytical column of your choice.Manufactured from PEEK material, the columns are free from corrosion and metal contamination and are packed with optimally sized, cation-exchange resin. The columns are attached with finger-tight fittings so no special spanners are required to ensure a leak-free seal. Additional analytical and prewash columns can be purchased. The Biochrom…
Specially designed for use with the Cecil Adept HPLC, Q-Adept HPLC, Merit HPLC and IonQuest ion chromatography.
A single quaternary low pressure mixing analytical pump, used in Q-Adept HPLC modular systems
A range of 0.01 to 50 ml/minute, used to create a high pressure mixing gradient.
The Accela 600 LC pump offers a maximum operating pressure of 600 bar and involves the use of unique and innovative Force Feedback Control (FFC), enabling the delivery of accurate and precise flow and gradients under all operating conditions. This pump is able to deliver unmatched compositional and flow rate accuracy and precision over the entire operating range resulting in exceptional reproducibility. Designed for configurat…
The Accela 1000 LC pump offers a maximum operating pressure of 15,000 psi and involves the use of unique and innovative Force Feedback Control (FFC), enabling the delivery of accurate and precise flow and gradients under all operating conditions. This pump is also able to deliver unmatched compositional and flow rate accuracy and precision over the entire operating range resulting in exceptional reproducibility making it an un…
A new member of the HyPURITY® family introduced at Pittcon 2003, HyPURITY AQUASTAR™ columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. The robust nature of the bonded phase results in a silica-based column that is unsurpassed for LC and LC/MS applications. This next generation column also offers enhanced perf…
The GPC Quattro is designed for the clean-up of complex animal tissues, food products and environmental samples by separating high molecular weight matrixes such as lipids, carbohydrates, pigments, etc., from analytical samples. Beneficial features of the GPC Quattro include: • Fourfold increase in productivity in comparison to traditional single-column instruments• High throughput • Economical price • Full error management…
UHPLC quaternary solvent delivery system with operating pressure to 1250 barThe Thermo Scientific Accela high-speed chromatographic system provides fast, efficient HPLC and U-HPLC in One Efficient, Limitless System. The Accela™ 1250 High Speed LC pump provides accurate and precise flow and gradients under all operating conditions with minimum pulsation. Features of the UHPLC Accela 1250 pump: • Quaternary capabilities with f…
Allows simpler, time saving methods for reversed phase chromatography of basic compounds: Purospher® RP-18 is a versatile HPLC column designed for the separation of problematic basic compounds with simple neutral eluents. This results in less time and cost because of simpler method optimization. Purospher® RP-18 is based upon a high purity, metal free silica. The multi-step chemical modification and deactivation of the surface…
Excellent peak symmetry with either basic or strongly acidic compounds: Purospher® RP-18 endcapped is a versatile HPLC column producing excellent peak shapes, designed for both the separation of basic compounds with simple neutral eluents and for the elution of strongly acidic compounds. Excellent separations in a shorter period of time with very good peak symmetry saves time and money. The excellent balance of Purospher® RP-1…
