PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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TaqPath respiratory viral select panel

Thermo Fisher Scientific

The Applied Biosystems TaqPath Respiratory Viral Select Panel is a multiplex, real-time PCR (qPCR) in vitro diagnostic (IVD) test for identifying five common respiratory viruses in under three hours. The CE-IVD panel includes targets for adenovirus, human metapneumovirus, rhinovirus/enterovirus,* and parainfluenza** virus. 

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  • Best New Clinical Assay or Kit of the Year

therascreen KRAS RGQ PCR Kit

QIAGEN

The  therascreen  KRAS RGQ PCR Kit is a real-time, qualitative in vitro diagnostic test for the detection of 7 somatic mutations in the  KRAS  oncogene using a sample of DNA extracted from formalin-fixed, paraffin-embedded (FPPE) non-small cell lung cancer (NSCLC) tissue and colorectal cancer (CRC) tissue.

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InviScreen® Norovirus Detection Kit

Invitek Diagnostics

The InviScreen ® Norovirus Detection Kit provides a real-time RT-PCR method for the qualitative detection of norovirus genogroups I (GI) and II (GII) in food samples. The method is based on ISO 15216 and detects specific regions in the conserved 5′ end of the ORF1-ORF2 junction. The method is applicable to test samples of foodstuffs and surface swabs. The kit includes Infectious Bronchitis Virus (IBV) as a process control vi…

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InviScreen® STEC Detection Kit

Invitek Diagnostics

The InviScreen ® STEC Detection Kit provides a real-time PCR method for the qualitative detection of STEC E. coli in food samples. The method is based on ISO 13136 and detects the presence of the stx1 and stx2 genes (associated with cytotoxicity) and the eae gene which is associated with the capability of the bacteria to adhere to the intestinal epithelium and cause disease. The method is applicable to products intended for h…

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InviScreen® Clostridium Botulinum Detection Kit

Invitek Diagnostics

The InviScreen ® Clostridium Botulinum Detection Kit is specifically designed for the qualitative detection of the specific botulinum neurotoxins (BoNTs) A, B, E and F of Clostridium botulinum in food, feed, and environmental samples. The real-time PCR procedure for detecting the presence of genes encoding BoNT type A (BoNT/A), type B (BoNT/B), type E (BoNT/E) and type F (BoNT/F) complies with the ISO/TS 17919 standard. The…

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InviScreen® GMO PCR Detection

Invitek Diagnostics

The InviScreen ® GMO Detection is a molecular biology tool that allows to easily detect genetically modified organisms (GMOs) using a fast, ISO 21569 standard-based, real-time PCR detection method based on the detection of the pCaMV35S, tNOS and pFMV genes. The kits can be used with a variety of starting materials, and benefits from the InviSorb® extraction kits that offer efficient protocols for DNA purification.

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InviScreen® Allergen PCR Detection

Invitek Diagnostics

The InviScreen ® Allergen Detection is a premium quality molecular biology tool to provide the general laboratory and research personnel an easy and ready-to-use kit for the detection of specific allergen species in food and feed samples. For DNA purification prior to testing, the Invisorb ® extraction kits offer efficient protocols for various starting materials. Benefit from our real-time PCR detection technology and get ac…

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Thermo Scientific Red Hot Taq DNA Polymerase

Thermo Fisher Scientific

Thermo Scientific Red Hot Taq DNA Polymerase consists of ThermoPrime Taq DNA Polymerase with an inert red dye to facilitate accurate low volume pipetting and as an indicator of enzyme addition. This dye has no adverse effect on the outcome of PCR; yields are the same as with standard ThermoPrime Taq DNA Polymerase. Highlights • Red colouring facilitates low volume pipetting • Reaction mix becomes pink on enzyme addition for ea…

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Pre-Aliquoted PCR Master Mix

Thermo Fisher Scientific

Thermo Fisher Scientific offers all ABgene PCR Master Mixes in a convenient pre-aliquoted format. This time-saving solution allows for more reproducible results by minimizing reagent handling steps and pipetting errors. Our Pre-aliquoted Master Mixes can be tailored to your very needs as custom primers can be added on request, and you can choose a plate or tube format that will suit your experimental set-up. Pre-aliquoted PCR…

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PCR Nucleotide Mix

This ready-to-use nucleotide mix is a premixed solution of multiple PCR-grade nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration of 10 mM). This mix is optimized for use in all types of amplification reactions and primer-extension reactions. PCR RT-PCR cDNA synthesis Primer extension Benefits Improve yield and performance Choose a convenient ready-to-use mix of all 4 nucleoti…

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Deoxynucleoside Triphosphate Set

The Deoxynucleoside Triphosphate Set contains 4 individual vials of dATP, dCTP, dGTP, and dTTP, each as a 100 mM solution of the Na-salt. PCR-grade nucleotides are optimized for use in all types of amplification reactions and primer-extension reactions. Product Description PCR-Grade Nucleotides from Roche Applied Science are specially manufactured and purified to the highest possible chemical purity. PCR-Grade Nucleoti…

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Transcriptor First Strand cDNA Synthesis Kit

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications: Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments. Generating cDN…

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Transcriptor High Fidelity cDNA Synthesis Kit

The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications: Cloning genes of inter…

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FastStart Universal SYBR Green Master (Rox)

FastStart Universal SYBR Green Master (Rox) includes a novel reference dye, which enables its use without modifications or adjustments to the specific instrument or protocol on all real-time PCR instruments requiring normalization with ROX. This ready-to-use, 2x-concentrated master mix contains all reagents (except primers and template) needed for running quantitative, real-time DNA detection assays, including qPCR and two-ste…

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FastStart Taq DNA Polymerase, dNTPack

The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides.FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incuba…

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FastStart High Fidelity PCR System, dNTPack

The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult…

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FastStart High Fidelity PCR System

The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult, challenging PCRs The FastStart High Fidelity PCR System is the product of choice for multiplex PCR. Please see the onl…

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GC-RICH PCR System, dNTPack

The enzyme dNTPack comprises the GC-RICH PCR System and a ready-to-use solution of PCR-Grade Nucleotides.The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior perfo…

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GC-RICH PCR System

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates. PCR Amplification of difficult templates up to 5 kb…

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Expand High FidelityPLUS PCR System, dNTPack

The enzyme dNTPack comprises the Expand High FidelityPLUS PCR System and a ready-to-use solution of PCR-Grade Nucleotides. Expand High FidelityPLUS PCR System is a next-generation blend consisting of Taq DNA Polymerase and a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself. Choose Expand High FidelityPLUS PCR Syste…

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Expand High Fidelity PCR System

The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length. PCR RT-PCR Benefits The E…

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Expand High Fidelity PCR System, dNTPack

The enzyme dNTPack comprises the Expand High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR…

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Pwo SuperYield DNA Polymerase, dNTPack

The enzyme dNTPack comprises the Pwo SuperYield DNA Polymerase and PCR-Grade Nucleotides in a ready-to-use solution.Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of…

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