Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3ʹ to 5ʹ exoribonuclease available for circular RNA cleanup, alternative splicing research, and gene expression studies. Key features: Efficacy and quality assured under ISO 13485 certification Available in bulk and for custom configurations Effectively degrades linear RNA, Y-structure RNAs, but not lariat loops or circular RNAs Digests linear RNAs…
96-well, thermal cycler for PCR with cables; for research use only.
Thermo Scientific Red Hot Taq DNA Polymerase consists of ThermoPrime Taq DNA Polymerase with an inert red dye to facilitate accurate low volume pipetting and as an indicator of enzyme addition. This dye has no adverse effect on the outcome of PCR; yields are the same as with standard ThermoPrime Taq DNA Polymerase. Highlights • Red colouring facilitates low volume pipetting • Reaction mix becomes pink on enzyme addition for ea…
Tube strips consist of eight 0.2 mL tubes connected together Cap strips are sold separately Made of thin wall polypropylene and designed for precise fit in heat blocks for optimum heat transfer Certified DNase- and RNase-free Autoclavable at 121°C
Thermo Fisher Scientific offers all ABgene PCR Master Mixes in a convenient pre-aliquoted format. This time-saving solution allows for more reproducible results by minimizing reagent handling steps and pipetting errors. Our Pre-aliquoted Master Mixes can be tailored to your very needs as custom primers can be added on request, and you can choose a plate or tube format that will suit your experimental set-up. Pre-aliquoted PCR…
This ready-to-use nucleotide mix is a premixed solution of multiple PCR-grade nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration of 10 mM). This mix is optimized for use in all types of amplification reactions and primer-extension reactions. PCR RT-PCR cDNA synthesis Primer extension Benefits Improve yield and performance Choose a convenient ready-to-use mix of all 4 nucleoti…
The Deoxynucleoside Triphosphate Set contains 4 individual vials of dATP, dCTP, dGTP, and dTTP, each as a 100 mM solution of the Na-salt. PCR-grade nucleotides are optimized for use in all types of amplification reactions and primer-extension reactions. Product Description PCR-Grade Nucleotides from Roche Applied Science are specially manufactured and purified to the highest possible chemical purity. PCR-Grade Nucleoti…
The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications: Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments. Generating cDN…
The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications: Cloning genes of inter…
FastStart Universal SYBR Green Master (Rox) includes a novel reference dye, which enables its use without modifications or adjustments to the specific instrument or protocol on all real-time PCR instruments requiring normalization with ROX. This ready-to-use, 2x-concentrated master mix contains all reagents (except primers and template) needed for running quantitative, real-time DNA detection assays, including qPCR and two-ste…
The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides.FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incuba…
The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult…
The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult, challenging PCRs The FastStart High Fidelity PCR System is the product of choice for multiplex PCR. Please see the onl…
The enzyme dNTPack comprises the GC-RICH PCR System and a ready-to-use solution of PCR-Grade Nucleotides.The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior perfo…
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates. PCR Amplification of difficult templates up to 5 kb…
The enzyme dNTPack comprises the Expand High FidelityPLUS PCR System and a ready-to-use solution of PCR-Grade Nucleotides. Expand High FidelityPLUS PCR System is a next-generation blend consisting of Taq DNA Polymerase and a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself. Choose Expand High FidelityPLUS PCR Syste…
The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length. PCR RT-PCR Benefits The E…
The enzyme dNTPack comprises the Expand High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR…
The enzyme dNTPack comprises the Pwo SuperYield DNA Polymerase and PCR-Grade Nucleotides in a ready-to-use solution.Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of…
The enzyme dNTPack comprises Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides. Taq DNA Polymerase is the recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus BM, expressed in E. coli. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease activity. PCR RT-PCR Primer…
DyNAmo™ Probe qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user.The 2x master mix includes the efficient hot start Thermus brockianus DNA polymerase. ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization. DyN…
Phusion™ RT-PCR Kit is a complete two-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps. In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase.In the second step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification…
Phusion™ Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of extremely short PCR protocols without compromising neither the fidelity nor the yield in the reaction. Phusion Flash DNA Polymerase used in the master mix is a proofreading DNA polymerase modified from Phusion Hot Start High-Fidelity DNA Polymerase. Phusion Flash High-Fidelity PCR Master Mix enables the use of extremely short cycling protocols w…
Finnzymes’ DyNAmo™ Flash SYBR® Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than other SYBR Green kits without compromising the qPCR performance.A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYB…
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications. This kit can be used in conjunction with Finnzymes' DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed…
