Western Blotting Products & Reviews

Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Abby™

Bio-Techne

Abby™ is the next generation chemiluminescence western protein analyzer. She automates protein size separation and immunodetection while delivering analyzed results of 24 samples in 3 hours. Abby saves the time spent in the lab carrying out traditional Western blot steps such as antibody additions, incubations, and washes. She delivers picogram-level sensitivity through chemiluminescence detection and workflow automation.

4.7/5.0
|1 Review



G-actin / F-actin in vivo assay kit

Cytoskeleton Inc.

The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions. The general approach is to homogenize cells in F-actin stabilization buffer, followed by centrifugation to separate the F-actin from G-actin pool. The fractions are then separate…

5.0/5.0
|1 Review