Western Blotting Products & Reviews

Polyclonal Rabbit antibody against SARS-CoV-2 (COVID-19) Spike Protein Subunit 1 Receptor Binding Domain

Aura® is the particle and aggregate detection system that combines Backgrounded Membrane Imaging which images 100% of your sample to give you count, size, and morphological information, with up to 2 channels of Fluorescence Membrane Microscopy. Identify if particles are protein or not with the first FMM channel and determine if your sample contains lipids, hydrophobic entities, or other aggregates of your choice with a second…

Aura® PTx gets you to your best formulation faster! Backgrounded Imaging (BMI) technology to give you accurate count, size, and morphology information about particles in your sample. Couple that with Fluorescence Membrane Microscopy (FMM), which gives you definitive ID of protein aggregates and an easy way to monitor polysorbate degradation.

Erasing storage phosphor screens made easy

Personal Western Blot Imager

The Amersham ImageQuant 500 imaging system is a compact, fast, easy-to-use CCD camera imaging system for chemiluminescence Western blotting, colorimetric, and UV fluorescence image capture.

Substrate-free immunoblot assay for extended signal stability

The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate.   The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. AttoPhos® Substrate (2′-[2-benzothiazoyl]-6′-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase to produce inorganic phosphate (Pi) and the alcohol, 2′-[2-benzothiazoyl]-6´-hydroxybenzothiazole (BB…

The Quick Western Kit provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step.

LICORbio Western Blotting Kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey infrared imaging systems.

When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. LICORbio offers primary antibodies for normalization that can be used for two-color normalization when performing multiplex Western blots or for cell-based assay normalization.

NewBlot IR Stripping Buffer is ideal for stripping and reprobing nitrocellulose and PVDF membranes detected with IRDye ® secondary antibodies. It may also be suitable for other dye-conjugated secondary antibodies.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700 channel.

Western Blot Storage Bags are ideal for long-term storage of near-infrared fluorescence Western blot membranes.

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

IRDye 680RD is the near-infrared fluorescent dye of choice for applications in the 700 nm wavelength, especially Western blots, cell-based assays such as the In-Cell Western assay, and small animal imaging experiments.

Odyssey One-Color Protein Molecular Weight Markers fluoresce in the 700nm channel of any of the Odyssey Imaging Systems and contain ten protein bands of 10, 15, 20, 25, 37, 50, 75, 100, 150, and 250 kDa, with molecular weight shifts of 5% and minimal band broadening.

IRDye Infrared Dyes and near-infrared (NIR) fluorescent imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region, therefore, giving a higher signal to noise ratios.

WesternSure chemiluminescent reagents offer the best performance available when compared to other competitive products on the market.

WesternSure ECL Stripping Buffer provides a robust solution for stripping primary and secondary antibodies from Western blot membranes while maintaining target antigen integrity for efficient reprobing.

Chameleon®  Pre-Stained Protein Ladders offer easy visualization of gel migration and protein size, and can be used to orient your gel or membrane quickly. These versatile protein markers offer multi-colored, pre-stained proteins for both visual inspection and two-color near-infrared detection. Choose from either single-channel or two-channel detection.

The WesternSure Protein Ladder is the only pre-stained, multi-colored protein ladder that provides chemiluminescent signal for film and digital detection. It will fit with your current protocol without optimization and does not rely on strep-tagged HRP or secondary antibody binding. The WesternSure protein ladder works with a variety of substrates and provides a vibrant color scheme that allows you to quickly orient your membr…

Chameleon Vue Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for molecular weight estimation

Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. It includes 11 protein bands from 8 to 260 kDa.