PCR and Thermal Cycling Products & Reviews

Brighter signal for earlier quantification of low and high copy RNA targets in a convenient 1-Step RT-qPCR format GoTaq® 1-Step RT-qPCR System is a reagent system that gives a brighter signal for earlier quantification of low and high copy RNA targets, using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube format. The system incorporates BRYT Green® Fluorescent Dye, a novel dye…

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples.   The kit contains: all 12 loci in the current PowerPlex® Y System, the additional 5 loci found in AmpFlSTR® Y-filer®, plus 6 new loci.   PowerPlex® Y23 System Benefits: Reveal more Y-STR loci in excess female DNA Cut your amplification time roughly in ha…

The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format.   It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add…

The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system protocol facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: • GoScript™ Reverse Transcription System • GoTaq® Probe qPCR Master Mix The GoScript™ Reverse Transcription System includes an optimized reaction b…

If there is DNA to detect, the Plexor® HY System will show it. Total human DNA. Total male DNA. The power to see it all in a single assay.   The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors…

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase.   This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…

All BRAND PCR products are available in white plastic, uniformly colored with titanium dioxide (TiO2) for optimal results when performing quantitative PCR (gPCR) with fluorescent signals.   BRAND microcentrifuge tubes fit most common rotors and may be spun up to 20,000 RCF (10,000 RCF for 0.5mL). Manufactured from virgin polypropylene, these highly translucent tubes are free from flash, air bubbles, and streaks. Caps are…

High quality PCR products for improved PCR efficiency.