Gene Expression and Molecular Cloning Products & Reviews

Our single-stranded DNA binding standard is a helix-destabilizing E. coli protein. The 75.6-kD protein is essential for replication, recombination, and repair processes.

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).  Detects protein-prote…

The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.  Tightly controlled T7 RNA polymerase-based gene expression in     E.coli    High-level expression upon induction  Convenien…

The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.  Tightly controlled T7 RNA polymerase-based gene expression in     E.coli    High-level expression upon induction  Convenien…

The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression. The establishment of a highly regulated expression system in eukaryotic cells is an invaluable tool for the study of cell cycle, oncogenicity, cytogenicity, and the mechanisms of gene regulation.

The Luciferase Assay Kit provides rapid, sensitive, and quantitative measurement of American firefly (Photinus pyralis) luciferase activity in cultured mammalian cells.  Sensitive, widely used reporter assay  Rapid, quantitative results  Reporter activity immediately available upon translation  Useful in conjunction with PathDetect systems

The LightSwitch promoter reporter GoClone collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 17,000 human promoters and highly optimized LightSwitch reagents. Measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminates the need for co-transfection…

An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…