Assay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.
SIRT1 belongs to the class III family of HDACs, which are NAD+ dependent enzymes. SIRT1 modulators have been shown to play a beneficial role in treating diseases such as cancer, diabetes, metabolic disease and inflammation, making them attractive targets for the development of new drugs. The new HTRF® SIRT1 assay, developed by Cisbio Bioassays using its patented HTRF technology, is a homogeneous method for directly measuring S…
With BI-DirectFlowTM flow injection technology and Gas Phase SPR, compatible with Electrochemistry SPR The BI-3000G SPR instrument is equipped for both liquid and vapor phase SPR analyses, and utilizes a unique detection technology that delivers high sensitivity (< 10-5 degrees) and fast response times (< 0.5 ms) with superior flexibility. The innovative use of interchangeable analysis modules is specially developed to fulfill…
High Performance Surface Plasmon Resonance (SPR) Instrument The BI-2000 SPR instrument produces superior, high-quality data at a completely affordable price. BI-SPR technology utilizes a unique detection technology that delivers high sensitivity and fast response times with superior versatility in a broad range of research applications. Our innovative use of interchangeable analysis modules is specially developed to fulfill a…
G-Protein Coupled Receptors (GPCRs) are common drug targets. Previously, most studies were based on the monomeric model, but this perception of GPCRs is now evolving towards homodimeric , heterodimeric and oligomeric models. To facilitate research into GPCR dimers, CisBio Bioassays has applied the Tag-lite® technology to GPCR interaction assays. Based on HTRF® technology, plasmids have been designed to express either the SNA…
Cisbio Bioassays offer a comprehensive cellular platform to develop HTRF® ligand binding assays.Features: • Single technology and cellular platform for all GPCR assays • Unique non-radioactive ligand binding assays • Miniaturization down to < 10µl • Peptidic and non-peptidic fluorescent ligands • High Specific Binding • Low Non-Specific Binding with HTRF technology Tag-lite fluorescent ligands available for (Feb10): Vasopre…
Tag-lite® cell-lines and reagents, a multimodal environment streamlined for GPCR studies. Cellular trans-membrane receptors are high potential therapeutic targets in pharmacological research, among them G-Protein Coupled Receptors (GPCRs). To reach a better physiological understanding, their study is deepening and is now focusing on structural assessments (ligand binding, dimerisation) and also on multiple signal pathway assa…
FasL-Strep, a stable homotrimer, efficiently induces the CD95 pathway of apoptosis and is highly suited for evaluation of CD95 (Fas) receptor-ligand interaction. Human or murine FasL-Strep (CD95L-T4) is an engineered human or murine Fas (CD95) ligand. C-terminal fusion of the T4-FOLDON to the extracellular FasL (CD95L) receptor-binding domain (E142-L281) results in bioactive, conformationally stabilized, well defined trimers…
Agilent’s contract Lead Discovery Service utilizes label-free RapidFire technology for direct quantitation of a wide range of native analytes across diverse therapeutic areas. Supports SAR programs via retesting of hits. Uses your existing assays or RapidFire custom-developed assays. Quick data turnaround for faster drug discovery decisions.Screened as singletons or compressed format The ideal candidate assays are often the sa…
Cell Membranes - Custom Preparation of Cell Membranes from CCS Cell Culture Service Preparations membranes from cell which are expressing a target receptor, transporter or ion channel at a high density are a useful tool in drug research. Embedded in a membrane fragment or vesicle the proteins remain in a functional native confirmation. Ligand binding assay and functional GTP-gammaS assays can be performed with these preparati…
