Transcreener® GDP GTPase Assays
BellBrook Labs Transcreener® GDP GTPase Assays
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The Bell Brook Labs Transcreener® GDP Assays are single step, homogenous, fluorescent assays for direct detection and screening of GTPases.
Available with fluorescence polarization (FP), fluorescence intensity (FI) and TR-FRET readouts, they provide a safe, HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than colorimetric phosphate detection methods. Based on BellBrook's proprietary nucleotide immmunodetection technology, the BellBrook Transcreener®GDP Assays are compatible with any enzyme class that produces GDP, including monomeric small G proteins, such as CDC42, and Gα subunits of heterotrimeric G proteins, such as Gαi1.
Features of BellBrook Labs Transcreener® GDP Assays:
- SENSITIVE: Highly selective, high affinity antibody means you can detect nanomolar GDP in the presence of 100-fold excess GTP
- UNIVERSAL: Use with any GDP-producing enzyme, at initial GTP concentrations of 0.1 to 1,000 μM.
- TURNKEY PROTOCOL: Run your enzyme reaction, add Transcreener detection reagents, read your plates.
- THREE DETECTION OPTIONS: Available in Fluorescence Polarization (FP), Fluorescence Intensity (FI) and TR-FRET detection modes that have been validated with major instrument platforms.
- VALIDATED HTS PLATFORM: Based on the Transcreener ADP2 Assay, which has been validated in over 40,000,000 wells of pharma HTS. See peer reviewed publications
