Gene Expression and Molecular Cloning Products & Reviews

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Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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pSG5 Vector

Agilent Technologies

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

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StrataClone Mammalian Expression Vector Systems

Agilent Technologies

The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.

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Vitality hrGFP & hrGFP II Mammalian Expression Vectors

Agilent Technologies

Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.

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Mycoplasma Plus PCR Primer Set

Agilent Technologies

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

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pCMV-Script Vectors

Agilent Technologies

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.

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GeneMorph II Random Mutagenesis Kits

Agilent Technologies

The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.

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GeneJammer Transfection Reagent

Agilent Technologies

The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.

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LipoTAXI Mammalian Transfection Reagent

Agilent Technologies

The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…

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MBS Mammalian Transfection Kit

Agilent Technologies

The MBS Mammalian Transfection kit can increase your transfection efficiencies up to 100-fold. Cells are incubated with CaPO 4 /DNA coprecipitate for only 3 hours, versus 6 to 12 hours with traditional CaPO 4 methods. Additionally, cells can be assayed 12 to 24 hours post-transfection instead of the usual 48 to 72 hours.  

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AAV Helper-Free Systems

Agilent Technologies

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

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AdEasy Adenoviral Vector Systems

Agilent Technologies

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

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pESC Yeast Epitope Tagging Vectors

Agilent Technologies

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.

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PiColorLock

Innova Biosciences Ltd.

Innova Biosciences, based on the Babraham Research Campus, Cambridge UK, has launched a family of assay reagents called PiColorLock™, to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzymes in microplates. Unlike the traditional "Malachite Green" method, which is prone to precipitation problems, PiColorLock reagent is specially formulated to give a stable end-point signal, meani…

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ColorKit

Innova Biosciences Ltd.

ColorKits are designed to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzynes in microplate assays. Each Kit contains a phosphorylated substrate for a particular class of enzyme, together with a carefully optimised PiColorLock detection reagent, Pi standard, 2x96-well plates and a selection of buffer concentrates for optimisation of assay conditions with the enzyme of interest.…

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PiColorLock Drug Discovery Kits

Innova Biosciences Ltd.

PiColorLock Drug Discovery Kits are designed to facilitate the measurement of the activity of potential inhibitors of phosphatases, ATPases and other Pi-generating enzymes in microplates. The Kits combine a purified recombinant human enzyme target, phosphorylated substrate, Pi standard and 2 multiwell plates. Each Kit is also supplied with a carefully optimised PiColorLock detection reagent and a selection of buffer concentr…

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AccuScript High-Fidelity RT-PCR Kit

Agilent Technologies

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

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AccuScript PfuUltra II RT-PCR Kit

Agilent Technologies

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

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AdEasy Viral Titer Kit

Agilent Technologies

The AdEasy Viral Titer Kit is a simple enzyme-linked immunoassay for the determination of adenoviral titers. The immunoassay detects an adenoviral capsid protein called hexon, which is required for adenoviral replication. The AdEasy viral titer kit can determine the titer of your stocks in 24 to 48 hours, compared to two weeks for traditional endpoint dilution assays.

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AdEasy XL Adenoviral System

Agilent Technologies

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

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AffinityScript One-Step RT-PCR Kit

Agilent Technologies

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C.

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