PCR and Thermal Cycling Products & Reviews

Thermo Scientific EasyStrips - Attached Cap Strip Tubes are designed for PCR and qPCR applications. They are compatible with 0.2 mL thermal cycler blocks and feature individually attached caps, for quick and easy access to samples. EasyStrips - Attached Cap Strip Tubes are provided with either Ultra Clear (for qPCR assays) or individual domed caps. This product is not available in the USA. Highlights • PCR strip tubes with ind…

Thermo Scientific ABsolute Fast QPCR Master Mix delivers highly reproducible, sensitive and specific detection of DNA and cDNA targets, using sequence-specific probes, under fast thermal cycling conditions. Features The ABsolute Fast QPCR Master Mix is an addition to the ABsolute Blue QPCR & Verso QRT-PCR product portfolio. Like both of these mixes, the ABsolute Fast QPCR Master Mix contains a pre-blended inert blue dye which…

Thermo Scientific Anchored Oligo-dT primers are specifically designed for synthesising long cDNAs from mRNAs carrying poly-A tails. Since this primer only anneals to the poly-A tail junction of the mRNA template, it eliminates transcription through the poly-A tail, providing more effective cDNA synthesis.

dNTP mixes – supplied in a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP. Thermo Scientific produces extremly pure dNTPs, and the company is one of only a few primary manufacturers in the industry. Thermo Scientific dNTPs are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually as nucleotide mixes at convenient concentrations for direct use in PCR, reverse transcription, label…

The Power SYBR® Green RNA-to-CT™ 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that are common in SYBR® Green-based assays. This significantly improves data accuracy and enables the reliable detection of low expressing targets or targets present at low amounts. Minimized hands-on time wit…

The SYBR Green RT-PCR Kit provides all the necessary components to perform both one- and two-step RT-PCR, which makes it ideal for evaluation between the two RT-PCR protocols.

The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…

The Veriti™ 384-Well Thermal Cycler (0.02 mL) delivers the proven reliability of Applied Biosystems 9700 GeneAmp® instruments with the throughput you need for large experiments. • Network up to 12 instruments together using standard Ethernet cables and a router (not provided) • From a single, networked Veriti™ instrument, start a run on up to 12 Veritis • Conveniently place instruments side-by-side due to front to back venti…

The Veriti™ 60-Well Thermal Cycler delivers the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® instruments with the 0.5 mL format you need to accomodate larger reaction volumes. . Additionally, the powerful user interface through the color touch screen simplifies the setup and use of the Veriti 60-Well Thermal Cycler. Optional setups for fast or standard PCR methods provide you the flexibility to shorten your…

Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex Primer Pools deliver comprehensive coverage of Sanger miRBase v10, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution. Optional Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs en…

Eppendorf real-time PCR Tube Strips feature an extremely thin wall for optimal heat transfer combined with high mechanical stability. In combination with their optical properties these are the ideal tube strips for any real-time PCR, especially for use with small reaction volumes. The Masterclear™ Cap Strips offer further advantages for your real-time PCR. The inverted dome of the Masterclear™ Cap Strips prevents scratching or…

Solaris qPCR Master Mix has been developed in conjunction with Solaris qPCR Gene Expression Assays for quantification of DNA and cDNA. Optimal qPCR results using probe detection chemistries can be achieved by using this same system of qPCR products. Optimized Solaris qPCR Master Mixes have been developed to ensure the best possible Solaris assay performance. In addition to enhanced performance, these optimized reagents offer…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated Hot Start PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DN…

The Freedom EVO PCR workstation is a fully scalable, modular system offering flexible automation for PCR set-up and cycle sequencing reaction preparation.

AutoDG™ Instrument for automated droplet generation of up to 96 samples, for use with the QX200™/QX100™ Droplet Digital™ PCR Systems  

5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100?  Droplet Digital™ PCR Systems

The ddSEQ Single-Cell Isolator is part of the Illumina® Bio-Rad® Single-Cell Sequencing Solution, a platform that brings Bio-Rad's Droplet Digital™ technology and Illumina's market-leading sequencing expertise to single-cell gene expression studies. The ddSEQ Single-Cell Isolator works with the Illumina® Bio-Rad® SureCell™ WTA 3' Library Prep Kit for the ddSEQ™ System. This technology utilizes disposable microfluidic cartri…

The SureCycler 8800 Thermal Cycler provides users with a complete package of market leading features and functionality. This PCR machine can run even the most complex thermal cycling techniques including time and temperature increments, touchdown PCR, and temperature gradients.

Qualified reagents for the polyadenylation of microRNAs (miRNAs) from total RNA and synthesis of first-strand cDNA from the tailed miRNAs.

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. The Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible—and reliable—instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies.Highly reproducible re…

The engineered PfuUltra II Fusion HS DNA Polymerase provides the worlds most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.  The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA  Dramatically reduced extension times allow f…

Brilliant II QRT-PCR reagents were developed to maintain the high quality of our original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations. With the addition of ROX reference dye to our master mix, we have streamlined the reaction setup process.