Enzyme Activity Assays Products & Reviews

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Enzyme Activity Assays

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DPPIV-Glo™ Protease Assay

Promega Corp.

The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. The DPPIV-Glo™ Assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a…

4.7/5.0
|1 Review
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High Sensitivity ß-Galactosidase Assay Kits

Agilent Technologies

The High Sensitivity ß-Galactosidase Assay Kit provides an easy, rapid, and sensitive method for determining the ß-galactosidase activity in the lysates of cells transfected with a ß-galactosidase expression construct. Rapid, simple and sensitive >= 10-fold increase in sensitivity over ONPG-based kits Reduces amount of cell extract needed for assay Useful for cells that are difficult to transfect and have low ß-gal expression…

5.0/5.0
|1 Review

Green Cathepsin B Assay

Immunochemistry Technologies LLC

ICT’s Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time in vitro using live whole cells.  This in vitro assay employs the quenched substrate Rhodamine 110-(RR)2, which fluoresces green upon cleavage by active cathepsin enzymes. Analyze samples using flow cytometry or fluorescence microscopy.

4.3/5.0
|4 Reviews
  • Best New Life Sciences Product of the Year

L-Lactate Assay Kit

Abcam plc

Key features and details Assay type: Quantitative Detection method: Colorimetric Platform: Microplate reader Assay time: 30 min Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine Sensitivity: 0.02 mM

3.7/5.0
|1 Review

Thermo Scientific™ Gallery™ Plus Enzyme Master Enzyme Analyzer

Thermo Fisher Scientific

Meet the Gallery Plus Enzyme Master - the first automated enzyme analyzer designed specifically for enzyme assay applications, with flexibility and automation Thanks to custom designed software, all reagent additions, incubations, and measurements are fully-automated, which saves time, eliminates manual error, and improves method robustness. And with reliable instrumentation, you can facilitate a simple method transfer from R…

0.0/5.0
|0 Reviews
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Thermo Scientific™ Gallery™ Enzyme Master Enzyme Analyzer

Thermo Fisher Scientific

Meet the Gallery Enzyme Master - the first automated enzyme analyzer designed specifically for enzyme assay applications. Combining robust hardware and new custom designed software, this best-in-class solution delivers fully-automated incubation settings, reagent additions, and precise measurement calculations—all with a touch of a button. This enzyme analyzer offers the smartest (and fastest) way yet to streamline method dev…

0.0/5.0
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Anti-CCP Assay

Svar Life Science AB

In diagnosis of Rheumatoid Arthritis (RA), the measurement of anti-CCP is integral. People who test positive for Anti-CCP, are very likely to develop or have already developed RA, but not all who have RA, necessarily develop anti-CCP. Early detection of RA is crucial for reducing tissue damage.

0.0/5.0
|0 Reviews
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Enzyme Modulators

Sino Biological

SignalChem(part of Sino Biological) offers one of the most comprehensive ranges of biologically active recombinant enzymes as well as a broad range of high-quality, low-cost enzyme activators and inhibitors of different classes of enzymes including protein kinases, phosphatases, and epigenetic enzymes.

0.0/5.0
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H2A.X Phosphorylation Assay Kit (Flow Cytometry)

Merck

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by…

0.0/5.0
|0 Reviews

TRAPeze® Telomerase Detection Kit

Merck

The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.

0.0/5.0
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TRAPeze® XL Telomerase Detection Kit

Merck

The TRAPEZE® XL Kit is a highly sensitive in vitro assay for the fluorometric detection of telomerase activity. As in the original TRAPEZE® Kit, primer sequence modifications that reduce amplification artifacts and an internal PCR control are included. In addition, the TRAPEZE® XL Kit uses fluorescence energy transfer (ET) primers to generate fluorescently labeled TRAP products which permit nonisotopic, quantitative analysis o…

0.0/5.0
|0 Reviews


Rho Assay Reagent (Rhotekin RBD, agarose)

Merck

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.

0.0/5.0
|0 Reviews

Rho Activation Assay Kit

Merck

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity 90%.

0.0/5.0
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Histone Deacetylase Assay Kit (HDAC)

Merck

This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.

0.0/5.0
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HDAC Assay Kit, fluorometric detection

Merck

This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.

0.0/5.0
|0 Reviews

Raf-1 Kinase Cascade Assay Kit

Merck

This kit is designed to measure the phosphotransferase activity in a kinase cascade reaction initiated by activated Raf-1. Recombinant unactive MEK1 is the substrate for Raf-1. Once activated by Raf-1, MEK1 can phosphorylate and thereby activate another kit component, recombinant unactive MAP Kinase 2/Erk2. Activation of MAP Kinase 2/Erk2 is determined by phosphorylation of myelin basic protein (MBP), in vitro. Phosphorylation…

0.0/5.0
|0 Reviews

B-Raf Kinase Cascade Assay Kit

Merck

The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.

0.0/5.0
|0 Reviews

B-Raf Kinase Assay Kit, Chemiluminescence Detection

Merck

Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user. For assay of B-Raf activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-B-Raf antibody (07-453).

0.0/5.0
|0 Reviews

Raf-1 Kinase Assay Kit, Chemiluminescence Detection

Merck

Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user. For assay of Raf-1 activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-Raf-1 antibody (07-396).

0.0/5.0
|0 Reviews

HDAC Assay Kit, fluorometric detection 384 well format

Merck

The 384 well format kit is a newer version of the tried and true non-radiographic HDAC assay. As in the previous kit, samples are first incubated with the HDAC Assay substrate, allowing deacetylation of the fluorometric substrate. After incubation, the activator solution is added, and releases a fluorophore from the deacetylated substrate or standard. The sample is then put into a microplate reader and fluorescence (i.e free/c…

0.0/5.0
|0 Reviews

HDAC Assay Kit, colorimetric detection

Merck

This assay is a simple two-step procedure performed in a microtiter plate. In the first step, samples are incubated with the HDAC assay substrate, allowing deacetylation of the substrate. Next, the Activator Solution releases p-nitroanilide from the deacetylated substrate or standard.

0.0/5.0
|0 Reviews