Immunoprecipitation Kits
GFP -Trap® - use your fluorescence fusion as tag for biochemical studies.Curiously GFP, the most widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited GFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses.Our GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matr…
Recombinant protein A covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein A agarose beads, protein A magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.
Magnetic Bead-Based Isolation of Proteins Millipore’s new PureProteome magnetic beads ensure the rapid and reproducible isolation of proteins. Unlike conventional methods that require centrifugation to pellet the beads then careful aspiration to avoid sample loss, PureProteome magnetic beads are isolated using a magnetic rack. This allows for the total removal of buffers for complete recovery of beads no sample dilution. You…
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been…
RFP -Trap® - use your fluorescence fusion as tag for biochemical studies Curiously RFP, a widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited RFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses. Superior results in a fraction of the time The ChromoTek RFP-Trap® overcomes these pro…
Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. Furthermore, GST-tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect GST fusion proteins. GST-Trap The GST-Trap is a highly specific and robust GST-binding protein coupled to ma…
DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…
Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles DNA and acts as a loading platform for multiple, transiently interacting factors participating in various DNA transactions. PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex n…
The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results. Features & Benefits • Complete set of materials for up to…
Purification Consumables and Custom Products.We understand that your purification challenges are unique. That’s why we offer you a variety of options to help you solve them. Whether you seek the up-front cost savings and versatility of packing your own columns, the consistent quality and time savings of pre-packed columns, or the ease associated with having us purify material for you; Sigma-Aldrich is your purification partner…
