Restriction Enzymes Products & Reviews

Restriction Enzyme

New Site-Specific Endoprotease that Targets Proline and Alanine

Presently, NEB supplies more than 200 restriction enzymes, over 120 of which are available in recombinant form.

Restriction enzyme digests are now even easier!The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format, including enzyme buffer and loading dye; simply add your DNA and digest for 15 minutes.Available Mixes: AgeI-HF® RE-Mix® OrderAscI RE-Mix® OrderBstEII-HF® RE-Mix® OrderEcoRI-HF® RE-Mix® OrderEcoRV-HF® RE-Mix® OrderKpnI-HF® RE-Mix® Or…

PvuII has a High Fidelity version PvuII-HF® (NEB #R3151) High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.  HF enzymes also exhibit dramatically reduced star activity.  HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without de…

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

Restriction Enzyme 

BbsI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119525.  NEB is excited to announce that all reaction buffers are now BSA-free. NEB began switching their BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

FastDigest® restriction enzymes are an advanced line of restriction enzymes for rapid DNA digestion. All FastDigest® enzymes are 100% active in the universal FastDigest® Green buffer and are able to digest DNA in 5-15 min. This enables any combination of restriction enzymes to mork simultaneously in one reaction tube and eliminates the need for sequential digestions. FastDigest® enzymes can be used to digest plasmid, genomica…

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.  Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart™ Buffer (over 215 enzymes are available in the same b…