A Novel 6x5 Peptide Mixture for LC-MS/MS Performance Monitoring

Performance monitoring of LC-MS/MS instrumentation continues to be challenging and has yet to be standardized across all MS laboratories. This poster reports the development of a novel reference peptide mixture and an accompanying software tool. The mixture reports on LC column performance, MS and MS/MS instrument parameters, including sensitivity and dynamic range. In addition, the software can report parameter history, compare instruments, and display XIC traces. This is the first example of a peptide mixture designed to give a report of all critical LC and MS parameters in a single run.