A novel RNA Detection Reagent Enables Gene Expression Analysis in Living Cells

Detecting gene expression has traditionally been limited to technologies that examine expression in lysed or fixed cell populations. The ability to detect RNAs in individual, live cells can enable an unequivocal assessment of gene expression changes that occur in direct response to specified perturbations. This application note demonstrates how the new SmartFlare™ detection reagent can be used for detecting specific mRNAs and miRNAs in live, intact cells. The applications of this novel technology are also discussed.