Analysis of Methylimidazoles in Caramel Colored Carbonated Beverages

This application note describes the use of an Ascentis Express HILIC column as a unique approach for analysis of the methylimidazole compounds. The direct analysis technique simplifies the sample workflow, and combined with HILIC chromatographic conditions, results in a simple and fast analytical method to determine the levels of 2-methylimidazole and 4-methylimidazole in caramel colored carbonated beverages.