Assessing Response of A431 Cells to Stimulation with Epidermal Growth Factor

This application note assesses the dose response of A431 cells (Human epithelial carcinoma cell line) to epithelial growth factor (EGF) as measured by specific antibodies detecting phosphorylated EGF receptor. Quantitative and simultaneous measurements of ERK and phosphorylation of EGF receptor in response to EGF stimulation were performed. A 96-well two-color In-Cell Western assay with the 800 and 700 channels detecting total EGF receptor (as normalization) and phosphorylated EGF receptor, respectively, was used.