Biophysical characterization of epigenetic protein interactions with chromatin using isothermal titration calorimetry

In this application note, Malvern Panalytical discuss how isothermal titration calorimetry (ITC) can be used to determine the contribution of individual protein residues or modifications to the total binding affinities of complex substrates. Incorporating binding, thermodynamic and stoichiometry data from ITC, structural studies like NMR and X-ray crystallography, and in vivo studies, will ultimately lead to a complete understanding of these complex interactions.