Chromapedia volume V: Applications

With the evolution of instruments, columns and cartridges, flash and prep HPLC has become the choice method of purification for an increasing number of applications. Because the compounds of interest and their properties vary so greatly, it is impossible to use a universal approach to perform separations of all sample types. Instead, long-lasting experience has helped define certain purification conditions that work best for each molecule group.

In this application note, Buchi discusses flash chromatography, prep HPLC, mobile and stationary phases, sample loading, and detection methods for each of these molecules:

• proteins/peptides
• vitamins, natural
• products/extracts
• lipids
• carbohydrates
• small molecule drugs