Comprehensive Determination of Trans Fats in Cookies using SP-2560 and SLB-IL111 GC Columns after Silver-Ion SPE Fractionation

This application note demonstrates the extraction of lipids as fatty acids, and subsequent detection of trans fats from a cookie sample made using partially hydrogenated vegetable oils. Ag-Ion SPE was used to fractionate the extracted sample into saturated FAMEs, and trans monoenes, cis monoenes, and dienes. Subsequent analysis of the monoene fractions on a highly polar SP-2560 column and an extremely polar SLB-IL111 column provided resolution of many of the C18:1 cis/trans geometricpositional isomers, with different elution patterns observed.