Convenient and reliable analysis of antibody drug conjugates

The addition of organic modifiers like isopropanol in hydrophobic interaction chromatography (HIC) can be an important parameter to decrease the retention of hydrophobic antibody drug conjugates (ADCs) as well as to adjust selectivity.

In this application note, Agilent demonstrates the drug-to-antibody (DAR) determination of brentuximab vedotin using a ternary gradient with isopropanol as organic modifier in the third channel on the Agilent 1260 Infinity II Prime Bio LC.