Extraction of Opiates From Human Urine Using ISOLUTE® SLE+ 96-Well Plates and Columns

This application note describes the extraction of a range of opiates from human urine using ISOLUTE SLE+ supported liquid extraction plates (96-well) and columns with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP) from human urine.