Fast Analysis of Isoflavones in Dietary Supplements – Method Transfer to an ACQUITY UPLC H-Class System

In this application note, the efficacy of different methods for the determination of isoflavones in dietary supplements was determined. The standard methods are based on reversed-phase high-performance liquid chromatography (RP-HPLC) and ultraviolet and visible light (UV-VIs) spectroscopy detection. By transferring a new isoflavones standard method onto the ACQUITY Arc UHPLC System, the run time of the analysis was cut from 70 minutes to 18 minutes.