High Sensitivity Measurement of Underivatized Estradiol by LC/MS/MS using Titan™ C18 Monodisperse Silica Columns

The sex hormone estradiol is crucial for the development, maintenance, and regulation of female secondary sex characteristics. In postmenopausal women, loss of estrogen is a principal factor in the development of osteoporosis. For the measurement of estradiol in human serum, a fast and robust LC/MS/MS method was developed using Titan C18 UHPLC columns.