Intelligent Time-Lapse Imaging to Enhance Live Cell Assay Development

The ability to monitor responses in living cells over a specific period of time offers cell biologists several key advantages for assay development. For routine cell based screening, time-course results can determine the correct time to read end-point assays. High content time-lapse imaging can also be used to characterize kinetics for multiple reactions at once, and to monitor cell proliferation or death. This application note demonstrates the performance of the ImageXpress® Micro Widefield High Content Screening System with an environmental control chamber, allowing live cells to be maintained over extended time periods within the instrument. In some cases, the system also performed scheduled fluidics addition or imaging of non-fluorescent cells with transmitted light.