Intra-Laboratory Validation of an Alternative Column Chemistry for the LC-MS/MS Analysis of Pesticides in Fruits and Vegetables Using Method EN 15662

LC-MS/MS methods are very popular for the analysis of pesticides in food. Several methods currently exist for their extraction from a variety of different food matrices. One of the newer methods is known as the QuEChERS, this method employs dispersive solid phase extraction (SPE) for extraction and cleanup. This method is now the European Norm (EN 15662). While this method typically uses C18 HPLC columns, this application note describes an intra-laboratory comparison and validation procedure using an Ascentis® Express RP-Amide column.