Measuring Cytochrome P450-3A4 Inhibition Assay on Hudson Robotics’ VaryScreen ADME Equipped with Berthold Technologies’ Mithras

Cytochrome P450-3A4 is an important enzyme for drug metabolism. In this application note the inhibition of Cytochrome P450-3A4 by various drug compounds is assayed using the VarySCreen ADME system and Mithras multimode reader. Inhibition of CYPs is reported using standard inhibition curves and IC 50 values.