Monitoring of T-cell invasions assay using a 3D spheroid model

In this application note, Molecular Devices describes a workflow for the generation of 3D tumor spheroids, co-cultured with T-cells as a proof-of-concept model for CAR T assays. Activated peripheral blood mononuclear cells (PBMCs) were added to spheroids and their activity monitored over time using high-content imaging. To optimize the workflow, Molecular Devices developed an image analysis approach that uses deep learning to accurately segment biological objects of interest and machine learning to quantify the T-cell induced phenotypic changes in the spheroids using only brightfield images.