Optimization of Wash/Elution Conditions for Automated Solid-Phase Extraction (SPE) of Testosterone from Plasma

This application note presents an automated SPE protocol based on the neutral polar polymeric SPE sorbent, Strata-X, which furnishes ultra-clean samples from plasma matrices. For comparison, results from a C18-E (hydrophobically end-capped) silica sorbent are also presented. It is inferred that this method will be applicable to steroids and hormones in general or other similar small molecules in a complex biological matrix.