Preparative chromatography focused gradients, pH control, and ionizable compounds

When applying a focused gradient after a scouting run, the desired compounds may not always elute at the expected time due to the effects of compound ionization and pH control. When using the PeakTrak^® Focused Gradient Generator, the calculation includes a wide margin for variance, which allows most compounds to elute during the focused gradient portion of the run even if the retention shifts due to equilibration of ionized and nonionized forms of the compound. The PeakTrak Focused Gradient Generator calculation is designed to place a peak in the middle of a focused gradient to allow for some error due to compound interconversion