Quantification of carbohydrates in various matrices

CAMAG highlights methods for the quantification of carbohydrates in various matrices using the HPTLC PRO System. The quantification of carbohydrates can be challenging due to their high polarity, low volatility, lack of a chromophore, and their common occurrence in complex matrices. The HPTLC PRO allows for the separation of mono- and oligosaccharides after minimal sample preparation and sensitive detection and quantification of these compounds after post-chromatographic derivatization. The note provides a method that can reliably and accurately quantify carbohydrates in different matrices using the HPTLC PRO System.