Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Milk using SPE Cleanup and LC/MS Analysis

Aflatoxins are produced by fungi and these species can infect important cereal grains, including maize, as well as ground (peanuts) and tree nuts. The focus of this study is the metabolite of AFB1, aflatoxin M1 (AFM1) that can be present in the milk of lactating dairy animals that have consumed aflatoxin contaminated feeds. A fast method for cleanup and analysis has been developed using Supel™ Tox AflaZea SPE, Ascentis® Express C18 HPLC column and LC/MS.