Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability

In this application note, a Schistosoma viability assay (96 and 384 well format) is introduced that utilises two fluorophores. Fluorescein diacetate emission is related to living cells while propidium iodide emission indicates the number of dead cells. This assay abandons low throughput microscopy and was performed in bottom reading mode on the POLARstar Omega microplate reader from BMG LABTECH.