Quantitative Imaging of Chemiluminescent Western Blots: Comparison of Digital Imaging and X-ray Film

Chemiluminescent western blotting is the most frequently used method for detecting proteins. Recently there has been a trend towards chemifluorescence which provides the user with many advantages including shorter exposure times and also means the signal is stable for weeks. This application note will compare the performance of x-ray film with that of Syngene’s G:BOX Chemi IR6 imaging system for the visualisation and quantification of chemiluminescent and chemifluorescence signals on western blots.