Save the Flavor — Robust Iso-α-Acids Assaying in Beer within Ten Minutes

This application note provides an instant result about the content of isohumulones in untreated beer within less than ten minutes, including sample preparation, HPLC separation, and result evaluation. Isohumulones (iso-α-acids) are derived by humulones (α-acids), essential constituents of hop resins. Iso-α- acids form approximately eighty percent of the typical bitterness of beer. Their antimicrobial effect leads to a sterile beverage, their tensioactive character stabilizes the foam, and they have a major influence on the general flavor, smell, and smoothness of beer.