Screening Cancer Genes Using NimbleGen Sequence Capture Arrays and the GS Junior Sequencing System

This application note by Roche illustrates a method for screening a core set of exons from 75 genes associated with cancer in a single GS Junior sequencing run. Oligo hybridization methods have been shown to effectively enrich for both contiguous and disjoint regions of the genome with much less effort than is required for PCR. DNA from three human cancer cell lines was captured and sequenced. Known variants from the literature were identified along with >900 previously unobserved variants including 30–60 insertions and deletions per sample.