Screening HDAC Inhibitors – A Workflow Comprising High Throughput and High Content Screening

To allow efficient screening of large compound libraries, intelligent screening workflows are needed that combine different assay formats and multiplex various readouts, including phenotypic information, to provide a more holistic view of compound effects. This application note demonstrates the potential power of combinatorial screening campaigns. An AlphaLISA assay was run on the EnVision Multilabel Plate Reader as a primary screen and a number of active compounds were then selected to be further analyzed and verified in a cellular assay using the Operetta High Content Imaging System.