Simultaneous Determination of Prescription and Designer Benzodiazepines in Urine by SPE and LC-MS/MS

Benzodiazepines are prescribed for the treatment of anxiety, insomnia, muscle spasms, alcohol withdrawal and seizure prevention. They are also recurrently utilized as illegal recreational drugs and, similar to other commonly abused compounds, “ legal” alternatives have been developed in an attempt to bypass the controlled substances act. This application note describes a method for the simultaneous detection of prescription and synthetic Benzodiazepine in urine using UCT’s Clean Screen^® XCEL I extraction columns in conjunction with the Selectra^®DA HPLC column.