StainFree Technology for Cellular Image Analysis without Fluorescent Dyes

This application note demonstrates how StainFree Technology enables cell counting that is comparable to nuclear and whole-cell counts obtained using fluorescent dyes. IC50 curves for compound-treated cells obtained through StainFree analysis are also presented as an alternative to using fluorescent reagents. Imaging cell-based assays typically requires the use of fluorescent probes that can be toxic to living cells or may only function in fixed cells. A label-free method for analyzing cell counts and cell confluence enables researchers to quantitatively monitor cell proliferation and health without time-consuming workflows that may disrupt cell viability.