TRP’ing on QPatch in Mulitmode Mode

In this application note three different transient receptor potential ion channels (TRPA1, TRPV1 & TRPM8) are tested in both single-hole mode and in multihole mode patch clamping with different agonists and antagonists. The advantages of testing compounds in multihole mode will be a minimization of biological variance and an increase in current amplitude, since the current response from several cells are summarized. The disadvantage is lack of serial resistance compensation and in some cases large leak currents.