Use of Red Firefly Luciferase to Monitor Infiltration of <i>Nicotiana benthamiana</i> for <i>In Planta</i> Bioluminescence

Luciferase reporter systems have been used extensively within in planta studies, most notably to study changes in the expression of transgenes. In this study, bioluminescence imaging (BLI) was applied to visualize the infection of a transgenic organism expressing a red-shifted luciferase into the leaves of a host plant, Nicotiana benthamiana.