Using the Sartobind® pico: Optimizing Steps for Using Micro-Scale Membrane Adsorbers on Liquid Chromatography Systems

One main difference between adsorbers and columns in sample preparation is related to the high loading capacity of membrane adsorbers. This means that a significantly large amount of sample passes through the adsorber without binding to the membrane while removing contaminating proteins, DNA, endotoxin and viruses. To reduce the sample consumption for laboratory experiments, Sartorius has introduced the Sartobind pico capsule which can be used to model contaminant removal, using a relatively small amount of sample. This application note provides a series of steps for optimization of the use of this device.