Using the UVP BioDoc-It Imaging System for Analysis of Differential Display RT-PCR Gels

Differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) is a technique in molecular biology, which allows the detection of genes, which are positively or negatively regulated transcriptionally under conditions of interest, without knowing the gene sequences in advance. This application note demonstrates that the UVP BioDoc-It system gives ethidium bromide staining 2-4 times the sensitivity of silver staining, with a much greater dynamic range.