Versatile Delivery of shRNA with High-Titer Lentivirus

This application note demonstrates the gene suppressing activity of the Lenti-X™ shRNA Expression System in a wide variety of cells and tissues. Recombinant lentiviruses are extremely efficient, versatile vectors that can permanently transduce genetic material into the genome of virtually any cell. Most cell types are susceptible to infection by VSV-G pseudotyped lentivirus, including dividing and non-dividing cells, neurons, stem cells, and primary cell cultures. These features make lentivirus an ideal vector for universal shRNA delivery.