Western Blot Normalization with Azure Biosystems

Normalization is critical for obtaining accurate, quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel, and for differences in transfer efficiency across a blot. Traditionally, protein normalization for Western blots involves comparing the relative abundance of a protein of interest to that of an unrelated protein, the normalization or loading control. This application note explains a total protein normalization (TPN) method, in which the relative abundance of the protein of interest is compared to the total protein content of the sample.