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<strong>Figure 1 Western Blot Validation of PUMA in K562 and 3T3/NIH Cells</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: 3041 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:1000

PUMA Antibody

Manufacturer: Prosci Inc.
Catalog Number: 3041
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: ELISA, Immunofluorescence, Immunohistochemistry (Formalin-Fixed / Paraffin-Embedded Sections), Western Blotting

<strong>Figure 1 Western Blot Validation of PUMA in K562 Cells</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: 3043 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution

PUMA Antibody

Manufacturer: Prosci Inc.
Catalog Number: 3043
Host: Rabbit

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag probed with 46-254 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-254 (1mg/ml) in Lane B. Primary

PUM2 Antibody

Manufacturer: Prosci Inc.
Catalog Number: 46-254
Host: Goat
Reactivity: Human
Applications: ELISA, Western Blotting

Western Blot: Pumilio-1 Antibody [NB100-259] - NIH-3T3 cell lysate (50 ug loaded), probed with Pumilio-1 antibody diluted at 0.1 ug/ml.

PUM1 Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB100-259
Host: Goat
Reactivity: Human, Mouse
Applications: Gel Super Shift Assay, Immunocytochemistry, Immunoprecipitation, Western Blotting

Immunohistochemistry: PUMA Antibody [NB100-56370] - PUMA expression in human dysplastic nevi (premalignant melanoma).

PUMA Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB100-56370
Host: Rabbit
Reactivity: Human, Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting

Western Blot: Pumilio 2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.

PUM2 Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB100-387
Host: Rabbit
Reactivity: Human, Mouse
Applications: Immunocytochemistry, Immunoprecipitation, Western Blotting

PUM1 Antibody

Manufacturer: Genesee Scientific
Catalog Number: 62-999
Host: Rat

PUM2 Antibody

Manufacturer: Genesee Scientific
Catalog Number: 62-998
Host: Rat

PUM2 Antibody

Manufacturer: Genesee Scientific
Catalog Number: 46-254
Host: Goat

PUM2 Antibody

Manufacturer: Genesee Scientific
Catalog Number: 63-175
Host: Rat