A step-by-step workflow for an HPRT knock-out experiment in an adherent cell line

This step-by-step tutorial video demonstrates the process of conducting an HPRT gene knock-out experiment in a HEK293 adherent cell line using Invitrogen™ Lipofectamine™ CRISPRMAX™. See how to design the experiment using the Invitrogen™ TrueDesign™ Genome Editor software; prepare both cells and reagents; conduct post-transfection assessment; perform genomic cleavage detection; and isolate and clonally expand the culture for further experimentation. For additional support designing CRISPR gene editing experiments, visit thermofisher.com/geneediting or explore the other tutorial videos in this series:

• A step-by-step workflow for a knock-in experiment in an adherent cell line >>
• A step-by-step workflow for a knock-out experiment in iPSCs >>
• A step-by-step workflow for a knock-out experiment in T cells >>